Rapid B Cell Linear Epitope Mapping
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چکیده
Conformational epitopes are thought to form the majority of strong antibody binding epitopes on most proteins. However numerous conformational epitopes may also be recognizable as linear epitopes, although with lower affinity. Linear B cell epitopes typically vary from 5 to 20 amino acids in length. If the antibody-binding region of the protein is unknown, an overlapping peptide library can be generated using a specific peptide length and offset, for example, 15mers offset by 5 amino acids (i.e. overlapping by 10).
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